Hair samples were daily irradiated for 10 h, using a mercury lamp full-spectrum (UV, VIS and IR), which was followed by a period in the dark (> 14 h), after which the sequence was repeated cyclically, until completing a total irradiation of 230 or 600 h. Two washing conditions were combined to the irradiation steps: 1. hair strands washed after each 10 h irradiation cycle (totalizing 23 or 60 washings in samples irradiated for 230 and 600 h, respectively), and 2. hair strands that were not washed, only irradiated. The effect of washing alone was evaluated in samples that were not irradiated, but only washed for 23 or 60 times.
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