I have never freezed my henna, but in my experience, after the henna has released, that it becomes "bad" quicker once you put it on your hair. I'm not sure if it's because of the heat on your scalp, or whatnot, because when I leave it in the sun, it will stay "active" for several hours. Once I put it on my hair, the color difference from 5 hours on isn't much different, so 5 seems to be the peak time of the dye's effectiveness. I use LUSH henna, though, which isn't pure, so straight henna might release differently.
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